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Example Input Mode:
Partner Data
CBIS
Matrix
Example Data Type:
Secondary Screen (Kd)
Primary Screen(% control or competition)
If the input file contains Primary and Secondary Data, use:
Primary
Secondary
Assay Data File:
.txt or .csv format - download examples:
Bromodomain
Kd
Primary
To highlight spots, select a compound(s), click 'fill' and select entries.
Create Images for:
R547
Highlight These Spots (
fill
):
R547|CAMK2A|2000
R547|CAMK2B|890
R547|CAMK2D|1400
R547|CAMK2G|1900
R547|CAMKK2|1400
R547|CASK|66
R547|CDC2L1|2300
R547|CDC2L2|2900
R547|CDC2L5|110
R547|CDK2|0.53
R547|CDK3|3.2
R547|CDK4-cyclinD1|0.61
R547|CDK5|7.4
R547|CDK7|0.58
R547|CDK9|15
R547|CDKL5|4000
R547|CLK1|66
R547|CLK2|80
R547|CLK3|13
R547|CLK4|310
R547|DCAMKL3|620
R547|DMPK|610
R547|DYRK1A|23
R547|DYRK1B|250
R547|ERK5|110
R547|ERK8|69
R547|GSK3A|280
R547|GSK3B|230
R547|ICK|2.2
R547|LOK|380
R547|MAK|11
R547|MAP3K1|3500
R547|MAP4K2|2900
R547|PCTK1|0.54
R547|PCTK2|0.86
R547|PCTK3|20
R547|PFTAIRE2|7.2
R547|PFTK1|11
R547|PIK4CB|1600
R547|PKN2|7300
R547|RIPK5|780
R547|ROCK1|2100
R547|ROCK2|730
R547|RSK3(Kin.Dom.2-C-terminal)|390
R547|SLK|96
R547|TAOK1|600
R547|TAOK2|750
R547|TAOK3|210
R547|TNNI3K|3800
R547|TTK|6200
R547|CAMK2A
R547|CAMK2B
R547|CAMK2D
R547|CAMK2G
R547|CAMKK2
R547|CASK
R547|CDC2L1
R547|CDC2L2
R547|CDC2L5
R547|CDK2
R547|CDK3
R547|CDK4-cyclinD1
R547|CDK5
R547|CDK7
R547|CDK9
R547|CDKL5
R547|CLK1
R547|CLK2
R547|CLK3
R547|CLK4
R547|DCAMKL3
R547|DMPK
R547|DYRK1A
R547|DYRK1B
R547|ERK5
R547|ERK8
R547|GSK3A
R547|GSK3B
R547|ICK
R547|LOK
R547|MAK
R547|MAP3K1
R547|MAP4K2
R547|PCTK1
R547|PCTK2
R547|PCTK3
R547|PFTAIRE2
R547|PFTK1
R547|PIK4CB
R547|PKN2
R547|RIPK5
R547|ROCK1
R547|ROCK2
R547|RSK3(Kin.Dom.2-C-terminal)
R547|SLK
R547|TAOK1
R547|TAOK2
R547|TAOK3
R547|TNNI3K
R547|TTK
R547|CAMK2A|2000
R547|CAMK2B|890
R547|CAMK2D|1400
R547|CAMK2G|1900
R547|CAMKK2|1400
R547|CASK|66
R547|CDC2L1|2300
R547|CDC2L2|2900
R547|CDC2L5|110
R547|CDK2|0.53
R547|CDK3|3.2
R547|CDK4-cyclinD1|0.61
R547|CDK5|7.4
R547|CDK7|0.58
R547|CDK9|15
R547|CDKL5|4000
R547|CLK1|66
R547|CLK2|80
R547|CLK3|13
R547|CLK4|310
R547|DCAMKL3|620
R547|DMPK|610
R547|DYRK1A|23
R547|DYRK1B|250
R547|ERK5|110
R547|ERK8|69
R547|GSK3A|280
R547|GSK3B|230
R547|ICK|2.2
R547|LOK|380
R547|MAK|11
R547|MAP3K1|3500
R547|MAP4K2|2900
R547|PCTK1|0.54
R547|PCTK2|0.86
R547|PCTK3|20
R547|PFTAIRE2|7.2
R547|PFTK1|11
R547|PIK4CB|1600
R547|PKN2|7300
R547|RIPK5|780
R547|ROCK1|2100
R547|ROCK2|730
R547|RSK3(Kin.Dom.2-C-terminal)|390
R547|SLK|96
R547|TAOK1|600
R547|TAOK2|750
R547|TAOK3|210
R547|TNNI3K|3800
R547|TTK|6200
clear
Select Highlight spots color from Pallette:
(also used in table view)
Tree Image Options
Restore default options
Spot options:
IndianRed
PaleGreen
Gold
PowderBlue
Fill?
Outline?
Outline thickness:
1
2
3
4
5
Select Spots Color from Pallette:
(spot highlight color below)
micro
nano
Spot Size:
Scaling Factor:
.1
.2
.3
.4
.5
.6
.7
.8
.9
1
1.1
1.2
1.3
Area increase factor:
100%
90%
80%
70%
60%
50%
(per change of 10x Kd or 10% Competition)
Legend options:
Left align Text
Right align Text
Label options:
Compound Name?
Compound Name Font:
.5
1
1.5
2
Show Assay Panel information?
Show Panel Spots?
Append Concentration? (i.e., 'AB500@1000nm'; check before loading.)
Background Image:
Target labels?
Dark Tree Branch image?
Hide Lipid, Mutant, ATypical and Pathogen?
Spot options:
Spot Color transparency level:
none
low
medium-low
medium
high
no color
Spot Cutoff Value:
Kd (nM) (Blank for no cutoff)
Spot outline only?
Format:
JPEG(200KB per image)
Publication Quality(5MB image)
Create Publication Quality (5MB bitmap) Image for:
Select Compound
R547
size/quality
100
90
80
70
60
50
40
30
% of original
White Space Border:
Yes
No
Preview and Highlighting
Highlight an assay by clicking on the spot or checking the box next to the Gene Symbol Name.
Click 'Update' to create the new images.
Compound To Highlight:
None
R547
Highlight Panel?
* WildType kinase assays appear in both the main TREEspot and Mutant kinase dendrogams.
Human Kinome image created by KINOMEscan.
2 images created. To download (copy and paste) all images to your local machine click Open/Browse Image Folder.
Images Saved To:
D:\Eurofins\PROD\Applications\FrontOffice_Discovery\StudyManagement\TreeSpot\HTSTools\TreeImages\538_wfydgfgkdy1idtduggmi1tcf\
Open/Browse Image Folder
View Options:
(These options do not modify the images that have been created, only the view on this page)
R547
Legend
Show Selected
Size:
Select Size
small
medium
full size
Images per Row:
1
Legend Resize?
Hover the cursor to identify spots and click to highlighted it. To save an image click the Zoom/Download link.
Zoom/Download
Highlight
Locate an Assay
CAMK2A
CAMK2B
CAMK2D
CAMK2G
CAMKK2
CASK
CDC2L1
CDC2L2
CDC2L5
CDK2
CDK3
CDK4-cyclinD1
CDK5
CDK7
CDK9
CLK1
CLK2
CLK3
CLK4
DCAMKL3
DMPK
DYRK1A
DYRK1B
ERK5
ERK8
GSK3A
GSK3B
ICK
LOK
MAK
MAP4K2
PCTK1
PCTK2
PCTK3
PFTAIRE2
PFTK1
PIK4CB
RIPK5
ROCK1
ROCK2
RSK3(Kin.Dom.2-C-terminal)
SLK
TAOK1
TAOK2
TAOK3
Legend
Data Table View:
Show All Data
Only Selected Compounds/Trees
TREE
spot
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